|
Characterization of transposable elements
(jumping genes) in the oak genome.
The study of
transposon evolution is an exciting area of research that has been understudied
in angiosperms. Transposons are mobile genetic elements that replicate via
reverse transcription or directly by a cut-and-paste mechanism.
Studies of plant transposons have shown that they are a very important
source genetic variation; yet, their diversity and their patterns of evolution
have been carried out in only a handful of plant species.
To achieve a better understanding of the evolutionary forces acting on
transposons, I am interested in identifying and characterizing them in the oak
genome. Once this information is
available, I intend to expand this study to other plant taxa, and to apply
methods of comparative genomics to gain insight into the underlying evolutionary
mechanisms of these DNA sequences. Using
a PCR-based approach, I have successfully isolated the Enhancer/Supressor-mutator
(En/Spm) transposable elements from oak species. Subsequent steps will involve the cloning, sequencing, and
DNA sequence analysis of these mobile elements.
Knowledge on the patterns and processes of transposons will enhance our
understanding of the evolution of plant genomes.
|